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p ire1α  (Novus Biologicals)


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    Novus Biologicals p ire1α
    P Ire1α, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ire1/IRE1+alpha+%5Bp+Ser724%5D+Antibody+-+BSA+Free/us12605424-306-40-42
    Average 96 stars, based on 241 article reviews
    p ire1α - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Radio Immunoprecipitation:

    Article Title: Attenuation of the upregulation of NF‑κB and AP‑1 DNA‑binding activities induced by tunicamycin or hypoxia/reoxygenation in neonatal rat cardiomyocytes by SERCA2a overexpression.
    Article Snippet: .. Primary antibodies against BiP (cat. no. 3183; 1:1,000), phospho‐PERK (cat. no. 3179; 1:1,000), PERK (cat. no. 3192; 1:1,000), phospho‐eIF2α (cat. no. 3398; 1:1,000), eIF2α (cat. no. 9722; 1:1,000), phospho‐NF‐κB p65 (Ser536) (cat. no. 3033; 1:1,000), NF‐κB p65 (cat. no. 8242; 1:1,000), SERCA2 (cat. no. 9580; 1:1,000) and histone H3 (cat. no. 4499; 1:2,000) were purchased from Cell Signaling Technology, Inc., those against phospho‐IRE1 (cat. no. ab48187; 1:1,000) and caspase‐12 (cat. no. ab62484; 1:500) were from Abcam, that against IRE1 (cat. no. NB100‐2324; 1:1,000) was from Novus Biologicals, LLc, that against cHOP (cat. no. sc‐7351; 1:200) was from Santa Cruz Biotechnology, Inc. and that against GAPDH (cat. no. 60004‐1‐Ig; 1:2,000) was from Proteintech Group, Inc. HRP‐conjugated secondary antibodies of goat anti‐mouse IgG (cat. no. sc‐2005; 1:3,000) and goat anti‐rabbit IgG (cat. no. sc‐2004; 1:3,000) were purchased from Santa cruz Biotechnology, Inc. Whole‐cell extracts were prepared using radioimmunoprecipitation assay lysis buffer (cat. no. CW2333; Beijing Cowinbioscience co., Ltd.) containing a protease inhibitor cocktail (cat. no. CW2200; Beijing Cowinbioscience Co., Ltd.) and phos‐ phatase inhibitors (cat. no. CW2383; Beijing Cowinbioscience co., Ltd.). cytoplasmic and nuclear extracts were prepared using the NE‐PERTM Nuclear and cytoplasmic Extraction Reagents (cat. no. 78833; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. .. The protein concentration in each sample was determined using the BcA Protein Assay kit (cat. no. CW0014; Beijing Cowinbioscience co., Ltd.) with bovine serum albumin as a standard.

    Article Title: Attenuation of the upregulation of NF-κB and AP-1 DNA-binding activities induced by tunicamycin or hypoxia/reoxygenation in neonatal rat cardiomyocytes by SERCA2a overexpression
    Article Snippet: The sample was electrophoresed on a 1% agarose gel in 0.5X Tris-borate-EDTA buffer at 120 V for 1.5 h, and then electrophoretically blotted onto a nylon membrane at 380 mA for 1 h. The membrane was cross-linked in a UV-light cross-linker (Analytik Jena AG) for 10 min, and the biotin-labeled DNA was detected by chemiluminescence. .. Primary antibodies against BiP (cat. no. 3183; 1:1,000), phospho-PERK (cat. no. 3179; 1:1,000), PERK (cat. no. 3192; 1:1,000), phospho-eIF2α (cat. no. 3398; 1:1,000), eIF2α (cat. no. 9722; 1:1,000), phospho-NF-κB p65 (Ser536) (cat. no. 3033; 1:1,000), NF-κB p65 (cat. no. 8242; 1:1,000), SERCA2 (cat. no. 9580; 1:1,000) and histone H3 (cat. no. 4499; 1:2,000) were purchased from Cell Signaling Technology, Inc., those against phospho-IRE1 (cat. no. ab48187; 1:1,000) and caspase-12 (cat. no. ab62484; 1:500) were from Abcam, that against IRE1 (cat. no. NB100-2324; 1:1,000) was from Novus Biologicals, LLC, that against CHOP (cat. no. sc-7351; 1:200) was from Santa Cruz Biotechnology, Inc. and that against GAPDH (cat. no. 60004-1-Ig; 1:2,000) was from Proteintech Group, Inc. HRP-conjugated secondary antibodies of goat anti-mouse IgG (cat. no. sc-2005; 1:3,000) and goat anti-rabbit IgG (cat. no. sc-2004; 1:3,000) were purchased from Santa Cruz Biotechnology, Inc. Whole-cell extracts were prepared using radioimmunoprecipitation assay lysis buffer (cat. no. CW2333; Beijing Cowinbioscience Co., Ltd.) containing a protease inhibitor cocktail (cat. no. CW2200; Beijing Cowinbioscience Co., Ltd.) and phosphatase inhibitors (cat. no. CW2383; Beijing Cowinbioscience Co., Ltd.). .. Cytoplasmic and nuclear extracts were prepared using the NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (cat. no. 78833; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions.

    Lysis:

    Article Title: Attenuation of the upregulation of NF‑κB and AP‑1 DNA‑binding activities induced by tunicamycin or hypoxia/reoxygenation in neonatal rat cardiomyocytes by SERCA2a overexpression.
    Article Snippet: .. Primary antibodies against BiP (cat. no. 3183; 1:1,000), phospho‐PERK (cat. no. 3179; 1:1,000), PERK (cat. no. 3192; 1:1,000), phospho‐eIF2α (cat. no. 3398; 1:1,000), eIF2α (cat. no. 9722; 1:1,000), phospho‐NF‐κB p65 (Ser536) (cat. no. 3033; 1:1,000), NF‐κB p65 (cat. no. 8242; 1:1,000), SERCA2 (cat. no. 9580; 1:1,000) and histone H3 (cat. no. 4499; 1:2,000) were purchased from Cell Signaling Technology, Inc., those against phospho‐IRE1 (cat. no. ab48187; 1:1,000) and caspase‐12 (cat. no. ab62484; 1:500) were from Abcam, that against IRE1 (cat. no. NB100‐2324; 1:1,000) was from Novus Biologicals, LLc, that against cHOP (cat. no. sc‐7351; 1:200) was from Santa Cruz Biotechnology, Inc. and that against GAPDH (cat. no. 60004‐1‐Ig; 1:2,000) was from Proteintech Group, Inc. HRP‐conjugated secondary antibodies of goat anti‐mouse IgG (cat. no. sc‐2005; 1:3,000) and goat anti‐rabbit IgG (cat. no. sc‐2004; 1:3,000) were purchased from Santa cruz Biotechnology, Inc. Whole‐cell extracts were prepared using radioimmunoprecipitation assay lysis buffer (cat. no. CW2333; Beijing Cowinbioscience co., Ltd.) containing a protease inhibitor cocktail (cat. no. CW2200; Beijing Cowinbioscience Co., Ltd.) and phos‐ phatase inhibitors (cat. no. CW2383; Beijing Cowinbioscience co., Ltd.). cytoplasmic and nuclear extracts were prepared using the NE‐PERTM Nuclear and cytoplasmic Extraction Reagents (cat. no. 78833; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. .. The protein concentration in each sample was determined using the BcA Protein Assay kit (cat. no. CW0014; Beijing Cowinbioscience co., Ltd.) with bovine serum albumin as a standard.

    Article Title: Attenuation of the upregulation of NF-κB and AP-1 DNA-binding activities induced by tunicamycin or hypoxia/reoxygenation in neonatal rat cardiomyocytes by SERCA2a overexpression
    Article Snippet: The sample was electrophoresed on a 1% agarose gel in 0.5X Tris-borate-EDTA buffer at 120 V for 1.5 h, and then electrophoretically blotted onto a nylon membrane at 380 mA for 1 h. The membrane was cross-linked in a UV-light cross-linker (Analytik Jena AG) for 10 min, and the biotin-labeled DNA was detected by chemiluminescence. .. Primary antibodies against BiP (cat. no. 3183; 1:1,000), phospho-PERK (cat. no. 3179; 1:1,000), PERK (cat. no. 3192; 1:1,000), phospho-eIF2α (cat. no. 3398; 1:1,000), eIF2α (cat. no. 9722; 1:1,000), phospho-NF-κB p65 (Ser536) (cat. no. 3033; 1:1,000), NF-κB p65 (cat. no. 8242; 1:1,000), SERCA2 (cat. no. 9580; 1:1,000) and histone H3 (cat. no. 4499; 1:2,000) were purchased from Cell Signaling Technology, Inc., those against phospho-IRE1 (cat. no. ab48187; 1:1,000) and caspase-12 (cat. no. ab62484; 1:500) were from Abcam, that against IRE1 (cat. no. NB100-2324; 1:1,000) was from Novus Biologicals, LLC, that against CHOP (cat. no. sc-7351; 1:200) was from Santa Cruz Biotechnology, Inc. and that against GAPDH (cat. no. 60004-1-Ig; 1:2,000) was from Proteintech Group, Inc. HRP-conjugated secondary antibodies of goat anti-mouse IgG (cat. no. sc-2005; 1:3,000) and goat anti-rabbit IgG (cat. no. sc-2004; 1:3,000) were purchased from Santa Cruz Biotechnology, Inc. Whole-cell extracts were prepared using radioimmunoprecipitation assay lysis buffer (cat. no. CW2333; Beijing Cowinbioscience Co., Ltd.) containing a protease inhibitor cocktail (cat. no. CW2200; Beijing Cowinbioscience Co., Ltd.) and phosphatase inhibitors (cat. no. CW2383; Beijing Cowinbioscience Co., Ltd.). .. Cytoplasmic and nuclear extracts were prepared using the NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (cat. no. 78833; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions.

    Protease Inhibitor:

    Article Title: Attenuation of the upregulation of NF‑κB and AP‑1 DNA‑binding activities induced by tunicamycin or hypoxia/reoxygenation in neonatal rat cardiomyocytes by SERCA2a overexpression.
    Article Snippet: .. Primary antibodies against BiP (cat. no. 3183; 1:1,000), phospho‐PERK (cat. no. 3179; 1:1,000), PERK (cat. no. 3192; 1:1,000), phospho‐eIF2α (cat. no. 3398; 1:1,000), eIF2α (cat. no. 9722; 1:1,000), phospho‐NF‐κB p65 (Ser536) (cat. no. 3033; 1:1,000), NF‐κB p65 (cat. no. 8242; 1:1,000), SERCA2 (cat. no. 9580; 1:1,000) and histone H3 (cat. no. 4499; 1:2,000) were purchased from Cell Signaling Technology, Inc., those against phospho‐IRE1 (cat. no. ab48187; 1:1,000) and caspase‐12 (cat. no. ab62484; 1:500) were from Abcam, that against IRE1 (cat. no. NB100‐2324; 1:1,000) was from Novus Biologicals, LLc, that against cHOP (cat. no. sc‐7351; 1:200) was from Santa Cruz Biotechnology, Inc. and that against GAPDH (cat. no. 60004‐1‐Ig; 1:2,000) was from Proteintech Group, Inc. HRP‐conjugated secondary antibodies of goat anti‐mouse IgG (cat. no. sc‐2005; 1:3,000) and goat anti‐rabbit IgG (cat. no. sc‐2004; 1:3,000) were purchased from Santa cruz Biotechnology, Inc. Whole‐cell extracts were prepared using radioimmunoprecipitation assay lysis buffer (cat. no. CW2333; Beijing Cowinbioscience co., Ltd.) containing a protease inhibitor cocktail (cat. no. CW2200; Beijing Cowinbioscience Co., Ltd.) and phos‐ phatase inhibitors (cat. no. CW2383; Beijing Cowinbioscience co., Ltd.). cytoplasmic and nuclear extracts were prepared using the NE‐PERTM Nuclear and cytoplasmic Extraction Reagents (cat. no. 78833; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. .. The protein concentration in each sample was determined using the BcA Protein Assay kit (cat. no. CW0014; Beijing Cowinbioscience co., Ltd.) with bovine serum albumin as a standard.

    Article Title: Attenuation of the upregulation of NF-κB and AP-1 DNA-binding activities induced by tunicamycin or hypoxia/reoxygenation in neonatal rat cardiomyocytes by SERCA2a overexpression
    Article Snippet: The sample was electrophoresed on a 1% agarose gel in 0.5X Tris-borate-EDTA buffer at 120 V for 1.5 h, and then electrophoretically blotted onto a nylon membrane at 380 mA for 1 h. The membrane was cross-linked in a UV-light cross-linker (Analytik Jena AG) for 10 min, and the biotin-labeled DNA was detected by chemiluminescence. .. Primary antibodies against BiP (cat. no. 3183; 1:1,000), phospho-PERK (cat. no. 3179; 1:1,000), PERK (cat. no. 3192; 1:1,000), phospho-eIF2α (cat. no. 3398; 1:1,000), eIF2α (cat. no. 9722; 1:1,000), phospho-NF-κB p65 (Ser536) (cat. no. 3033; 1:1,000), NF-κB p65 (cat. no. 8242; 1:1,000), SERCA2 (cat. no. 9580; 1:1,000) and histone H3 (cat. no. 4499; 1:2,000) were purchased from Cell Signaling Technology, Inc., those against phospho-IRE1 (cat. no. ab48187; 1:1,000) and caspase-12 (cat. no. ab62484; 1:500) were from Abcam, that against IRE1 (cat. no. NB100-2324; 1:1,000) was from Novus Biologicals, LLC, that against CHOP (cat. no. sc-7351; 1:200) was from Santa Cruz Biotechnology, Inc. and that against GAPDH (cat. no. 60004-1-Ig; 1:2,000) was from Proteintech Group, Inc. HRP-conjugated secondary antibodies of goat anti-mouse IgG (cat. no. sc-2005; 1:3,000) and goat anti-rabbit IgG (cat. no. sc-2004; 1:3,000) were purchased from Santa Cruz Biotechnology, Inc. Whole-cell extracts were prepared using radioimmunoprecipitation assay lysis buffer (cat. no. CW2333; Beijing Cowinbioscience Co., Ltd.) containing a protease inhibitor cocktail (cat. no. CW2200; Beijing Cowinbioscience Co., Ltd.) and phosphatase inhibitors (cat. no. CW2383; Beijing Cowinbioscience Co., Ltd.). .. Cytoplasmic and nuclear extracts were prepared using the NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (cat. no. 78833; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions.

    Extraction:

    Article Title: Attenuation of the upregulation of NF‑κB and AP‑1 DNA‑binding activities induced by tunicamycin or hypoxia/reoxygenation in neonatal rat cardiomyocytes by SERCA2a overexpression.
    Article Snippet: .. Primary antibodies against BiP (cat. no. 3183; 1:1,000), phospho‐PERK (cat. no. 3179; 1:1,000), PERK (cat. no. 3192; 1:1,000), phospho‐eIF2α (cat. no. 3398; 1:1,000), eIF2α (cat. no. 9722; 1:1,000), phospho‐NF‐κB p65 (Ser536) (cat. no. 3033; 1:1,000), NF‐κB p65 (cat. no. 8242; 1:1,000), SERCA2 (cat. no. 9580; 1:1,000) and histone H3 (cat. no. 4499; 1:2,000) were purchased from Cell Signaling Technology, Inc., those against phospho‐IRE1 (cat. no. ab48187; 1:1,000) and caspase‐12 (cat. no. ab62484; 1:500) were from Abcam, that against IRE1 (cat. no. NB100‐2324; 1:1,000) was from Novus Biologicals, LLc, that against cHOP (cat. no. sc‐7351; 1:200) was from Santa Cruz Biotechnology, Inc. and that against GAPDH (cat. no. 60004‐1‐Ig; 1:2,000) was from Proteintech Group, Inc. HRP‐conjugated secondary antibodies of goat anti‐mouse IgG (cat. no. sc‐2005; 1:3,000) and goat anti‐rabbit IgG (cat. no. sc‐2004; 1:3,000) were purchased from Santa cruz Biotechnology, Inc. Whole‐cell extracts were prepared using radioimmunoprecipitation assay lysis buffer (cat. no. CW2333; Beijing Cowinbioscience co., Ltd.) containing a protease inhibitor cocktail (cat. no. CW2200; Beijing Cowinbioscience Co., Ltd.) and phos‐ phatase inhibitors (cat. no. CW2383; Beijing Cowinbioscience co., Ltd.). cytoplasmic and nuclear extracts were prepared using the NE‐PERTM Nuclear and cytoplasmic Extraction Reagents (cat. no. 78833; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. .. The protein concentration in each sample was determined using the BcA Protein Assay kit (cat. no. CW0014; Beijing Cowinbioscience co., Ltd.) with bovine serum albumin as a standard.

    Western Blot:

    Article Title: Tumour suppressor protein sMEK1 links to IRE1 signalling pathway to modulate its activity during ER stress.
    Article Snippet: The Endoplasmic Reticulum is a pervasive, dynamic cellular organelle that performs a wide range of functions in the eukaryotic cell, including protein folding and maturation.. Upon stress, ER activates an adaptive cellular pathway, namely Unfolded Protein Response, that transduces information from ER to nucleus, restoring homeostasis in the ER milieu.. UPR consists of three membrane-tethered sensors; IRE1, PERK and ATF6.

    other:

    Article Title: Recovery of FAM134A-mediated ER-phagy through BRD4 inhibition alleviates ethanol-induced neurodegeneration
    Article Snippet: The antibodies of mCherry (NBP2-25157), IRE1 (NB100-2324), p-IRE1 (Ser724) (NB100-2323), and FAM134C (NBP1-60104) were purchased from Novus Biologicals.

    Staining:

    Article Title: Ammonia induces amyloidogenesis in astrocytes by promoting amyloid precursor protein translocation into the endoplasmic reticulum
    Article Snippet: Protein samples were subjected to SDS-PAGE and transferred to supported nitrocellulose membranes (GE Healthcare Life Sciences). .. Membranes were stained with ponceau S (Sigma-Aldrich) for protein detection, then blocked with blocking buffer (5% bovine serum albumin in Tris Buffered Saline with Tween 20) and probed with primary antibodies against APP (abcam, Y188), β-actin (Sigma-Aldrich, Clone AC-15), α-tubulin (Sigma-Aldrich, T5168), GM130 (BD Biosciences, Clone 35), PDI (Cell Signaling, C81H6), IRE1 (Novus biological, NB100-2324), phosphor-IRE1 (Novus biological, NB1002323), OASIS (Santa Cruz Biotechnology, sc-514635), presenilin-1 (Santa Cruz Biotechnology, sc-365450), BACE1 (Santa Cruz Biotechnology, sc-33711). .. Membranes were then probed with horseradish peroxidase–conjugated secondary antibodies (GE Healthcare) and visualized by ECL (SuperSignal West Dura Extended Duration Substrate, Thermo Fisher Scientific).

    Blocking Assay:

    Article Title: Ammonia induces amyloidogenesis in astrocytes by promoting amyloid precursor protein translocation into the endoplasmic reticulum
    Article Snippet: Protein samples were subjected to SDS-PAGE and transferred to supported nitrocellulose membranes (GE Healthcare Life Sciences). .. Membranes were stained with ponceau S (Sigma-Aldrich) for protein detection, then blocked with blocking buffer (5% bovine serum albumin in Tris Buffered Saline with Tween 20) and probed with primary antibodies against APP (abcam, Y188), β-actin (Sigma-Aldrich, Clone AC-15), α-tubulin (Sigma-Aldrich, T5168), GM130 (BD Biosciences, Clone 35), PDI (Cell Signaling, C81H6), IRE1 (Novus biological, NB100-2324), phosphor-IRE1 (Novus biological, NB1002323), OASIS (Santa Cruz Biotechnology, sc-514635), presenilin-1 (Santa Cruz Biotechnology, sc-365450), BACE1 (Santa Cruz Biotechnology, sc-33711). .. Membranes were then probed with horseradish peroxidase–conjugated secondary antibodies (GE Healthcare) and visualized by ECL (SuperSignal West Dura Extended Duration Substrate, Thermo Fisher Scientific).

    Saline:

    Article Title: Ammonia induces amyloidogenesis in astrocytes by promoting amyloid precursor protein translocation into the endoplasmic reticulum
    Article Snippet: Protein samples were subjected to SDS-PAGE and transferred to supported nitrocellulose membranes (GE Healthcare Life Sciences). .. Membranes were stained with ponceau S (Sigma-Aldrich) for protein detection, then blocked with blocking buffer (5% bovine serum albumin in Tris Buffered Saline with Tween 20) and probed with primary antibodies against APP (abcam, Y188), β-actin (Sigma-Aldrich, Clone AC-15), α-tubulin (Sigma-Aldrich, T5168), GM130 (BD Biosciences, Clone 35), PDI (Cell Signaling, C81H6), IRE1 (Novus biological, NB100-2324), phosphor-IRE1 (Novus biological, NB1002323), OASIS (Santa Cruz Biotechnology, sc-514635), presenilin-1 (Santa Cruz Biotechnology, sc-365450), BACE1 (Santa Cruz Biotechnology, sc-33711). .. Membranes were then probed with horseradish peroxidase–conjugated secondary antibodies (GE Healthcare) and visualized by ECL (SuperSignal West Dura Extended Duration Substrate, Thermo Fisher Scientific).



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    Mettl18 deficiency induces unfolded protein response (UPR) activation and inflammatory cytokine expression. (A–D) Quantification of UPR pathway activation. Western blot analysis of whole pancreatic lysates from WT and KO mice using (A) anti-PERK (phospho-Thr980) and anti-PERK antibodies to assess PERK phosphorylation; (B) anti-EIF2 (phospho-Ser51) and anti-EIF2 antibodies to assess EIF2 phosphorylation; (C) anti-ATF4 antibody; and (D) anti-IRE1 (phospho-Ser724) and anti-IRE1 antibodies. Band intensities were quantified with ImageJ. 16-week-old; n = 6; mean ± SEM. Student's t -test: p∗∗<0.01, p∗<0.05. See also . (E) qPCR quantification of Xbp1s mRNA levels. Ct values were normalized to those obtained with primers detecting both Xbp1s and unspliced Xbp1 ( Xbp1u ) as described in . 16-week-old; n = 7; mean ± SEM. Student's t -test: n.s. > 0.1. See also . (F and G) qPCR quantification of mRNA expression of inflammatory cytokines, Il-1 β (E) and Il-6 (F). 16-week-old; n = 7; mean ± SEM. Student's t -test: p∗<0.05. (H) IL-1β protein levels were assessed by Western blotting of whole pancreatic lysates from WT and KO mice using an anti-IL-1β antibody. Quantification and statistical analyses were performed as in (A–D). (I) Schematic representation of UPR activation in WT and Mettl18 KO pancreas.

    Journal: Molecular Metabolism

    Article Title: METTL18 ensures pancreatic function by maintaining proper translation and proteostasis

    doi: 10.1016/j.molmet.2026.102337

    Figure Lengend Snippet: Mettl18 deficiency induces unfolded protein response (UPR) activation and inflammatory cytokine expression. (A–D) Quantification of UPR pathway activation. Western blot analysis of whole pancreatic lysates from WT and KO mice using (A) anti-PERK (phospho-Thr980) and anti-PERK antibodies to assess PERK phosphorylation; (B) anti-EIF2 (phospho-Ser51) and anti-EIF2 antibodies to assess EIF2 phosphorylation; (C) anti-ATF4 antibody; and (D) anti-IRE1 (phospho-Ser724) and anti-IRE1 antibodies. Band intensities were quantified with ImageJ. 16-week-old; n = 6; mean ± SEM. Student's t -test: p∗∗<0.01, p∗<0.05. See also . (E) qPCR quantification of Xbp1s mRNA levels. Ct values were normalized to those obtained with primers detecting both Xbp1s and unspliced Xbp1 ( Xbp1u ) as described in . 16-week-old; n = 7; mean ± SEM. Student's t -test: n.s. > 0.1. See also . (F and G) qPCR quantification of mRNA expression of inflammatory cytokines, Il-1 β (E) and Il-6 (F). 16-week-old; n = 7; mean ± SEM. Student's t -test: p∗<0.05. (H) IL-1β protein levels were assessed by Western blotting of whole pancreatic lysates from WT and KO mice using an anti-IL-1β antibody. Quantification and statistical analyses were performed as in (A–D). (I) Schematic representation of UPR activation in WT and Mettl18 KO pancreas.

    Article Snippet: Other antibodies used were as follows: anti-β-actin (clone 6D1, cat#M177-3; MBL); anti-phospho-PERK (Thr980) (clone G.305.4, cat#MA5-15033; Invitrogen); anti-RPL3 (cat#66130-1-Ig), anti-Reg1 (cat#15850-1-AP), and anti-PERK/EIF2AK3 (cat#24390-1-AP) (Proteintech Group); anti-IRE1 (cat#3294), anti-ATF4 (cat#11815), and anti-eIF5A (cat#20765) (Cell Signaling Technology); anti-phospho-IRE1 (cat#ab48187), anti-phospho-EIF2A (cat#ab32157), and anti-EIF2A (cat#ab5369) (Abcam); anti-IL-1β (cat#AF-401-NA; R&D Systems).

    Techniques: Activation Assay, Expressing, Western Blot, Phospho-proteomics